DOT Rule 49 CFR Part 40 Section 40.82

Subpart F - Drug Testing Laboratories

§ 40.82 What drugs do laboratories test for?

As a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test “DOT specimens” for any other drugs. 

     (a) Marijuana metabolites. 

     (b) Cocaine metabolites. 

     (c) Amphetamines. 

     (d) Opioids. 

     (e) Phencyclidine (PCP). 

[82 FR 52244, Nov. 13, 2017. Redesignated at 88 FR 27643, May 2, 2023]