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Ask Us A Question

"Have a Question … Ask DOT!"

DOT Agency Regulations

If you have questions regarding:

  • Safety-Sensitive Positions
  • Pre-employment Tests
  • Post-Accident Tests
  • Random Tests
  • Reasonable Suspicion Tests
  • Return to Duty Tests
  • Follow-up Tests
  • Employee Prohibitions and Rule Violations
  • Employer Policy Development
  • Supervisor Training
  • Employee Training and Educational Materials
  • Recordkeeping, MIS and Other Reporting Requirements

Click on this link to find the appropriate drug and alcohol program office to send your DOT Agency questions.

49 CFR Part 40

If you have questions regarding:

  • Requirements of Obtaining New Employee Testing Records
  • Urine Specimen Collections
  • Drug Testing Laboratories
  • Medical Review Officers
  • Alcohol Testing
  • Substance Abuse Professionals
  • Return to Duty Process
  • Service Agent Responsibilities and Qualifications
  • Recordkeeping and Confidentiality Requirements

Click here to send the Office of Drug and Alcohol Policy and Compliance your Part 40 questions.

NOTE: Response time to e-mails will vary depending on the volume of e-mails and the complexity of the request.


A red and white checkered hot-air balloon is rising up toward the right, above a complex maze below. A slogan to the right of the balloon says, “Preventing Substance Abuse Begins with Knowledge.”

Last updated: Monday, October 29, 2018