DOT Rule 49 CFR Part 40 Section 40.199
Subpart I—Problems in Drug Tests
§ 40.199 What problems always cause a drug test to be cancelled?
(b) The following are “fatal flaws”:
(1) There is no CCF;
(2) In cases where a specimen has been collected, there is no specimen submitted with the CCF;
(3) There is no printed collector's name and no collector's signature;
(4) Two separate collections are performed using one CCF;
(5) The specimen ID numbers on the specimen bottle and the CCF do not match;
(6) The specimen bottle seal is broken or shows evidence of tampering (and a split specimen cannot be re-designated, see §40.83(h)); or
(7) Because of leakage or other causes, there is an insufficient amount of specimen in the primary specimen bottle for analysis and the specimens cannot be re-designated (see §40.83(h))
(8) For an oral fluid collection, the collector used an expired device at the time of collection.
(9) for an oral fluid collection, the collector failed to enter the expiration date in Step 4 of the CCF nd the laboratory confirmed that the device was expired.