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Urine Specimen Collection Guidelines

DOT Urine Specimen Collection Guidelines for the U.S. Department of Transportation Workplace Drug Testing Programs (49 CFR Part 40)

Revised October 1, 2010 [previous editions become obsolete].

These guidelines apply only to employers and individuals who come under the regulatory authority of the U.S. Department of Transportation (DOT) and those individuals who conduct urine specimen collections under DOT regulations. The term “employee” is used throughout this document and has the same meaning as “donor” as used on the Federal Drug Testing Custody and Control Form (CCF).