Official US Government Icon

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure Site Icon

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Drug Testing

This page shows Department of Transportation content pertaining to a specific tag. If you cannot find what you’re looking for on this page, please use the search box in the top right of our website.

DOT Rule 49 CFR Part 40 Section 40.93

Subpart F - Drug Testing Laboratories § 40.93 What validity tests must laboratories conduct on primary oral fluid specimens?

As a laboratory, if you conduct validity testing under § 40.92, you must conduct it in accordance with the requirements of this section. 

(a) You...

DOT Rule 49 CFR Part 40 Section 40.91

Subpart F - Drug Testing Laboratories § 40.91 What are the cutoff concentrations for oral fluid drug tests?

As a laboratory, you must use the cutoff concentrations displayed in the following table for initial and confirmatory drug tests for oral fluid specimens. All cutoff concentrations...

DOT Rule 49 CFR Part 40 Section 40.89

Subpart F - Drug Testing Laboratories § 40.89 What are the adulterant cutoff concentrations for initial and confirmation urine tests?

(a) As a laboratory, you must use the cutoff concentrations for the initial and confirmation adulterant testing as required by the HHS Mandatory Guidelines...

DOT Rule 49 CFR Part 40 Section 40.87

Subpart F - Drug Testing Laboratories § 40.87 What validity tests must laboratories conduct on primary urine specimens?

As a laboratory, when you conduct validity testing under § 40.86, you must conduct it in accordance with the requirements of this section. 

(a) You must...

DOT Rule 49 CFR Part 40 Section 40.85

Subpart F - Drug Testing Laboratories § 40.85 What are the cutoff concentrations for urine drug tests?

(a) As a laboratory, you must use the cutoff concentrations displayed in the following table for initial and confirmatory drug tests. All cutoff concentrations are expressed in...

DOT Rule 49 CFR Part 40 Section 40.83

Subpart F - Drug Testing Laboratories § 40.83 How do laboratories process incoming specimens?

As the laboratory, you must do the following when you receive a DOT specimen:

(a) You are authorized to receive only Copy 1 of the CCF. You are not authorized to receive other copies of...

DOT Rule 49 CFR Part 40 Section 40.81

Subpart F - Drug Testing Laboratories § 40.81 What laboratories may be used for DOT drug testing?

(a) As a drug testing laboratory located in the U.S., you are permitted to participate in DOT drug testing only if you are certified by HHS under the National Laboratory Certification Program...

DOT Rule 49 CFR Part 40 Section 40.73

Subpart E - Specimen Collections § 40.73 How is an oral fluid specimen collected?

(a) The collector must be present and maintain visual contact with the employee during the procedures outlined in this section. 

As the oral fluid collector, you must not allow any other person...