DOT's Notice on Testing for Marijuana
DOT OFFICE OF DRUG AND ALCOHOL POLICY AND COMPLIANCE NOTICE
On December 18, 2025, the President of the United States issued an Executive Order directing the...
DOT Drug Testing
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HHS Certified Oral Fluid Laboratories and Oral Fluid Collection Devices
This page contains a list of oral fluid drug testing laboratories certified by HHS under the National Laboratory Certification Program (NLCP). To comply with DOT’s drug testing regulation, 49 CFR Part 40, DOT-regulated employers may only utilize these laboratories and oral fluid devices. ...
Oral Fluid Collectors
How Can I Become an Oral Fluid Collector For DOT Drug Testing?
Oral Fluid Specimen Collection Procedures Guidelines
List of HHS certified oral fluid laboratories and oral fluid devices acceptable for DOT drug testing
eCCF...
'Back to Basics' for Medical Review Officers
As an MRO you have an important and indispensable role in the DOT drug testing process. You act as an independent and impartial 'gatekeeper' and you advocate for the accuracy and integrity of the drug testing process. Your quality assurance reviews of the drug testing process strengthen...
DOT Rule 49 CFR Part 40 Section 40.92
Subpart F - Drug Testing Laboratories § 40.92 What is oral fluid validity testing, and are laboratories required to conduct it?(a) Specimen validity testing is the evaluation of the specimen to determine if it is consistent with normal human oral fluid. The purpose of validity testing is...
DOT Rule 49 CFR Part 40 Section 40.90
Subpart F - Drug Testing Laboratories § 40.90 What criteria do laboratories use to establish that a urine specimen is invalid?(a) As a laboratory, you must use the invalid test result criteria for the initial and confirmation testing as required by the HHS Mandatory Guidelines, and you...
DOT Rule 49 CFR Part 40 Section 40.88
Subpart F - Drug Testing Laboratories § 40.88 What criteria do laboratories use to establish that a urine specimen is dilute or substituted?(a) As a laboratory, you must consider the primary specimen to be dilute when:
The creatinine concentration is greater than or...
DOT Rule 49 CFR Part 40 Section 40.86
Subpart F - Drug Testing Laboratories § 40.86 What is urine validity testing, and are laboratories required to conduct it?(a) Specimen validity testing is the evaluation of the specimen to determine if it is consistent with normal human urine. The purpose of validity testing is to...
CCF Notice (August 2021)
--- Reminder ---
The Revised Federal Drug Testing Custody and Control Form
must be used beginning August 30th, 2021[1]
Important Dates
We will conform to the dates HHS has...
DOT 5 Panel Notice
DOT Drug Testing: After January 1, 2018 – Still a 5-Panel
The DOT testing at HHS-certified laboratories is a 5-panel drug test regimen. As of January 1, 2018, the ‘Opiates’ category was renamed ‘Opioids’:
Marijuana (THC)CocaineAmphetaminesOpioidsPhencyclidine (PCP)Under ‘...